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KMID : 0616120180490010028
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Korean Journal of Pharmacognosy
2018 Volume.49 No. 1 p.28 ~ p.39
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Effect of Snail Extract on Bone Growth in Vitro and in Vivo
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Sohn Gee-Ho
Kim Tae-Hee
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Abstract
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This study investigated the effect of snail extract on the growth parameters of old female rats (27 weeks). Rats were
administered orally with snail extract at a dose of 100 mg/kg, 200 mg/kg, chondroitin sulfate 10 mg/kg and 0.9% saline (control) for 8 weeks. Bone mineral density (BMD) and serum concentrations of insulin-like growth factor 1 (IGF-1) and insulinlike growth factor-binding protein 3 (IGFBP-3) were significantly higher in rats exposed to snail extract for 8 weeks. MG-63 cells (human osteoblast-like cells) were treated with snail extract for 48 h. Their differentiation and proliferation was investigated with Western blot and morphological changes observed via immunofluorescence staining of β-catenin. Treatment with snail extract significantly increased the levels of growth factors including β-catenin and IGF-1. The snail extract affected osteoblast formation. Morphological changes in MG-63 cells were observed via immunofluorescence staining. Treatment with snail extract increased the expression of β-catenin in MG-63 cells. Results suggest that the treatment of MG-63 cells with snail extract increased the longitudinal growth and growth factor levels. Snail extract may be pharmacologically effective in osteogenic differentiation in vitro and represents a potential therapeutic agent for bone formation.
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KEYWORD
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Snail , Fruticiola sieboldiana , Bone growth
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